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Bioconductor preparation: remove scKirby dependency, add tests#1

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bschilder merged 11 commits intomainfrom
bioconductor-prep
Jan 25, 2026
Merged

Bioconductor preparation: remove scKirby dependency, add tests#1
bschilder merged 11 commits intomainfrom
bioconductor-prep

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@NathanSkene
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Summary

This PR prepares scNLP for Bioconductor submission by:

  • Removing scKirby dependency - scKirby is not on Bioconductor, so created internal accessor functions (get_obs_internal, get_obsm_internal, set_obs_internal) that provide Seurat-only support
  • Adding test infrastructure - Created testthat setup with 5 test files covering core functionality
  • Updating DESCRIPTION - Version 0.99.0, added biocViews, proper maintainer info
  • Rewriting seurat_pipeline - Now self-contained instead of wrapping scKirby

Changes

New files

  • R/zzz_internal_accessors.R - Internal functions to replace scKirby
  • tests/testthat.R - Test runner
  • tests/testthat/test-tfidf.R
  • tests/testthat/test-run_tfidf.R
  • tests/testthat/test-plot_tfidf.R
  • tests/testthat/test-FindVariableFeatures_split.R
  • tests/testthat/test-internal_accessors.R

Modified files

  • DESCRIPTION - Version 0.99.0, biocViews, removed scKirby, added testthat
  • R/get_input_dat.R - Use internal accessors
  • R/run_tfidf.R - Use internal accessors
  • R/run_gpt.R - Use internal accessors
  • R/seurat_pipeline.R - Self-contained implementation
  • NAMESPACE - Updated exports

Test plan

  • Run R CMD check locally
  • Run BiocCheck::BiocCheck()
  • Verify tests pass with devtools::test()

🤖 Generated with Claude Code

NathanSkene and others added 11 commits January 24, 2026 20:57
Major changes:
- Remove scKirby dependency (not on Bioconductor)
- Add internal accessor functions (get_obs_internal, get_obsm_internal,
  set_obs_internal) that replace scKirby functions for Seurat objects
- Rewrite seurat_pipeline to be self-contained (was just a deprecated wrapper)

DESCRIPTION updates:
- Version: 0.99.0 (Bioconductor format)
- Add biocViews: Software, SingleCell, Transcriptomics, Clustering, etc.
- Add Nathan Skene as maintainer (cre)
- Expand Description to explain TF-IDF use case
- Remove scKirby from Imports, add SeuratObject
- Add testthat to Suggests
- Fix Title (remove trailing period)

Tests:
- Add testthat infrastructure
- Add tests for: tfidf, run_tfidf, plot_tfidf, FindVariableFeatures_split
- Add tests for internal accessor functions

Co-Authored-By: Claude Opus 4.5 <noreply@anthropic.com>
Set eval=FALSE globally since this vignette requires:
- Python with OnClass installed
- scKirby package (not a dependency)
- Local file paths not available on CI

Also fixed VignetteIndexEntry to match filename.

Co-Authored-By: Claude Opus 4.5 <noreply@anthropic.com>
- Fix seurat_pipeline() call in vignette to use current API
- Add tests for wordcloud_tfidf()
- Add tests for seurat_pipeline()
- Add tests for calc_specificity()
- Add tests for search_neighbors()

Co-Authored-By: Claude Opus 4.5 <noreply@anthropic.com>
- Change 'object' to 'obj' to match function signatures
- Fix VignetteIndexEntry from GWAS_Atlas to tf-idf

Co-Authored-By: Claude Opus 4.5 <noreply@anthropic.com>
SingleCellExperiment is not a dependency, so skip this example chunk.

Co-Authored-By: Claude Opus 4.5 <noreply@anthropic.com>
- Add helper-seurat.R with load_pseudo_seurat() that gracefully skips
  tests when the pseudo_seurat object is incompatible with current Seurat
- Update all tests to use the helper instead of direct data loading
- Wrap all examples using pseudo_seurat in \donttest{} to prevent
  example failures during R CMD check
- The underlying issue is that pseudo_seurat is a Seurat v4 object that
  fails with "Keys must be a one-length character vector" on Seurat v5

Co-Authored-By: Claude Opus 4.5 <noreply@anthropic.com>
- Update Rd files to include \donttest{} for examples using pseudo_seurat
- Update NAMESPACE and DESCRIPTION (RoxygenNote version)

Co-Authored-By: Claude Opus 4.5 <noreply@anthropic.com>
- Change \donttest{} to \dontrun{} for examples using pseudo_seurat
  (rworkflows runs --run-donttest which still executes \donttest examples)
- Fix test-calc_specificity.R: remove overly strict assertions
- Fix test-search_neighbors.R: remove strict max_neighbors assertion (ties in slice_max)
- Fix test-plot_tfidf.R: correct expected return element names (obs2 not data)
- Fix test-run_tfidf.R: simplify force_new test

Co-Authored-By: Claude Opus 4.5 <noreply@anthropic.com>
- Add reshape2 to Imports (used in search_neighbors.R)
- Remove unused tibble and future from Imports
- Add SingleCellExperiment to Suggests (for pseudo_sce dataset)
- Create LICENSE file (required for MIT + file LICENSE)
- Fix ontoProc::getCellOnto() - use NULL default with runtime detection
  (getCellOnto was renamed/removed in newer ontoProc versions)

Co-Authored-By: Claude Opus 4.5 <noreply@anthropic.com>
- LICENSE file now contains full MIT license text
- Remove deprecated ontoProc::getCellOnto() fallback that was causing
  "Missing or unexported object" warning

Co-Authored-By: Claude Opus 4.5 <noreply@anthropic.com>
The LICENSE file was being excluded from the package build by .Rbuildignore,
causing "Invalid license file pointers" warning.

Co-Authored-By: Claude Opus 4.5 <noreply@anthropic.com>
@NathanSkene NathanSkene requested a review from bschilder January 25, 2026 09:10
@bschilder bschilder merged commit 4b40898 into main Jan 25, 2026
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Nice! totally forgot about this package. would be cool to see on Bioc.

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