Metabolic drivers of drug sensitivity in cancer cell lines

Introduction

To reveal the contribution of metabolism to drug sensitivity, we screened 31 drugs across 21 cancer cell lines at 5 logs of concentration, generating over 34.000 metabolic profiles coupled to 1 Mio images of over 3000 treatment groups as shown in Figure 1. From these results, we provide a map of drug-metabolite interactions that are general across many cell lines, several being independent of drug growth effect, a known confounder in metabolomics analysis. We discovered 703 drug-metabolite interactions, 488 of them being involved resistance and 215 in sensitivity. Of those, 599 are independent of drug growth effect – i.e. happens prior to growth effects in the concentration domain.

Figure 1. Graphical abstract of methods for sample processing in the paper.

Installation

Install required package confluencer:

 if(!require(confluencer)) {
  devtools::install_github("mauromiguelm/confluencer")
}

From the terminal: start myproject.Rproj

Metabolomics: coming soon

About

I am currently working towards documenting and publishing.