Value Errors

[hrehmaan]

ValueError: too many values to unpack (expected 3, got 4)

File "C:\Users\hrehm\Downloads\test\venv\Lib\site-packages\mps_motion\gui.py", line 132, in
pagespage
~~~~~~~~~~~^^
File "C:\Users\hrehm\Downloads\test\venv\Lib\site-packages\mps_motion\gui.py", line 78, in motion_analysis
u = opt_flow.get_displacements(scale=scale)
File "C:\Users\hrehm\Downloads\test\venv\Lib\site-packages\mps_motion\motion_tracking.py", line 262, in get_displacements
data = scaling.resize_data(self.data, scale)
File "C:\Users\hrehm\Downloads\test\venv\Lib\site-packages\mps_motion\scaling.py", line 50, in resize_data
new_frames = resize_frames(data.frames, scale)
File "C:\Users\hrehm\Downloads\test\venv\Lib\site-packages\mps_motion\scaling.py", line 116, in resize_frames
w, h, num_frames = frames.shape
^^^^^^^^^^^^^^^^

ValueError: Image must have 1, 2, 3 or 4 channels

File "C:\Users\hrehm\Downloads\test\venv\Lib\site-packages\mps_motion\gui.py", line 132, in
pagespage
~~~~~~~~~~~^^
File "C:\Users\hrehm\Downloads\test\venv\Lib\site-packages\mps_motion\gui.py", line 32, in video
mps.utils.frames2mp4(
~~~~~~~~~~~~~~~~~~~~^
data.frames.T,
^^^^^^^^^^^^^^
path=f.name,
^^^^^^^^^^^^
framerate=data.framerate,
^^^^^^^^^^^^^^^^^^^^^^^^^
)
^
File "C:\Users\hrehm\Downloads\test\venv\Lib\site-packages\mps\utils.py", line 441, in frames2mp4
imageio.mimwrite(mp4path, normalize_frames(frames), fps=framerate)
~~~~~~~~~~~~~~~~^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
File "C:\Users\hrehm\Downloads\test\venv\Lib\site-packages\imageio\v2.py", line 495, in mimwrite
return file.write(ims, is_batch=True, **kwargs)
~~~~~~~~~~^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
File "C:\Users\hrehm\Downloads\test\venv\Lib\site-packages\imageio\core\legacy_plugin_wrapper.py", line 253, in write
writer.append_data(image, metadata)
~~~~~~~~~~~~~~~~~~^^^^^^^^^^^^^^^^^
File "C:\Users\hrehm\Downloads\test\venv\Lib\site-packages\imageio\core\format.py", line 589, in append_data
return self._append_data(im, total_meta)
~~~~~~~~~~~~~~~~~^^^^^^^^^^^^^^^^
File "C:\Users\hrehm\Downloads\test\venv\Lib\site-packages\imageio\plugins\ffmpeg.py", line 590, in _append_data
raise ValueError("Image must have 1, 2, 3 or 4 channels")

[finsberg]

The error is happening because the frames in the dataset does not have the expected shape. If you open the file with with mps and print the shape

import mps

data = mps.MPS("file.czi")
print(data.frames.shape)

it will most likely be a tuple with more than three values. The library expects this to be (width, height, num_frames).