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bamxx.hpp
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266 lines (217 loc) · 6.3 KB
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/* MIT License
*
* Copyright (c) 2023 Andrew D Smith and Masaru Nakajima
*
* Permission is hereby granted, free of charge, to any person obtaining a copy
* of this software and associated documentation files (the "Software"), to deal
* in the Software without restriction, including without limitation the rights
* to use, copy, modify, merge, publish, distribute, sublicense, and/or sell
* copies of the Software, and to permit persons to whom the Software is
* furnished to do so, subject to the following conditions:
*
* The above copyright notice and this permission notice shall be included in
* all copies or substantial portions of the Software.
*
* THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR
* IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY,
* FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE
* AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER
* LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM,
* OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE
* SOFTWARE.
*/
#ifndef BAM_RECORD_HPP
#define BAM_RECORD_HPP
#include <htslib/bgzf.h>
#include <htslib/hfile.h>
#include <htslib/sam.h>
#include <htslib/thread_pool.h>
#include <algorithm>
#include <cstdlib>
#include <iterator> // IWYU pragma: keep
#include <limits>
#include <stdexcept>
#include <string>
#include <utility>
#include <sys/types.h> // for off_t
namespace bamxx {
struct bam_rec {
bam_rec() = default;
bam_rec(const bam_rec &other) : b{bam_copy1(bam_init1(), other.b)} {}
auto
operator=(bam_rec rhs) -> bam_rec & {
std::swap(b, rhs.b);
return *this;
}
~bam_rec() {
if (b != nullptr)
bam_destroy1(b);
}
bam1_t *b{};
};
struct bam_in {
explicit bam_in(const std::string &fn) : f{hts_open(fn.data(), "r")} {}
~bam_in() {
if (f != nullptr)
hts_close(f);
}
operator bool() const { return f != nullptr; }
template <typename T>
auto
read(T &h, bam_rec &b) -> bool {
if (b.b == nullptr)
b.b = bam_init1();
const int x = sam_read1(f, h.h, b.b); // -1 on EOF; args non-const
// ADS: (todo) get rid of exception
if (x < -1)
throw std::runtime_error("failed reading bam record");
return x >= 0;
}
auto
is_mapped_reads_file() const -> bool {
const htsFormat *fmt = hts_get_format(f);
return fmt->category == sequence_data &&
(fmt->format == bam || fmt->format == sam);
}
samFile *f{};
};
struct bam_header {
bam_header() = default;
bam_header(const bam_header &rhs) : h{bam_hdr_dup(rhs.h)} {}
explicit bam_header(bam_in &in) : h{sam_hdr_read(in.f)} {}
~bam_header() {
if (h != nullptr)
bam_hdr_destroy(h);
}
operator bool() const { return h != nullptr; }
auto
add_pg_line(const std::string &cmd, const std::string &id,
const std::string &vn) -> bool {
return sam_hdr_add_line(h, "PG", "ID", id.c_str(), "VN", vn.c_str(), "CL",
cmd.c_str(), nullptr) == 0;
}
auto
tostring() const -> std::string {
return sam_hdr_str(h);
}
sam_hdr_t *h{};
};
struct bam_out {
explicit bam_out(const std::string &fn, const bool fmt = false)
: f{hts_open(fn.c_str(), fmt ? "bw" : "w")} {}
~bam_out() {
if (f != nullptr)
hts_close(f);
}
operator bool() const { return f != nullptr; }
auto
write(const bam_header &h, const bam_rec &b) -> bool {
return sam_write1(f, h.h, b.b) >= 0;
}
auto
write(const bam_header &h) -> bool {
return sam_hdr_write(f, h.h) == 0;
}
htsFile *f{};
};
struct bgzf_file {
bgzf_file(const std::string &fn, const std::string &mode)
: f{bgzf_open(fn.c_str(), mode.c_str())} {}
~bgzf_file() { destroy(); }
operator bool() const { return f != nullptr; }
auto
write(const std::string &s) -> bool {
const auto res = bgzf_write(f, s.c_str(), s.size());
return static_cast<size_t>(res) == s.size();
}
auto
write(const char *const s, const size_t expected_size) -> bool {
const auto res = bgzf_write(f, s, expected_size);
return static_cast<size_t>(res) == expected_size;
}
auto
tellg() const -> off_t { // off_t is POSIX
return f == nullptr ? std::numeric_limits<off_t>::max() : htell(f->fp);
}
auto
destroy() -> void {
if (f != nullptr) {
bgzf_close(f);
f = nullptr;
}
}
bool
is_compressed() const {
// 0=no_compression, 1=gzip, 2=bgzf defined in the enum
// htsCompression from hts.h
return bgzf_compression(f) > 0;
}
bool
is_bgzf() const {
// 0=no_compression, 1=gzip, 2=bgzf defined in the enum
// htsCompression from hts.h
return bgzf_compression(f) == 2;
}
BGZF *f{};
};
inline auto
getline(bgzf_file &file, std::string &line) -> bgzf_file & {
if (file.f == nullptr)
return file;
kstring_t s{0, 0, nullptr};
const int x = bgzf_getline(file.f, '\n', &s);
if (x == -1) {
file.destroy();
line.clear();
}
else {
line.resize(s.l);
std::copy(s.s, s.s + s.l, std::begin(line));
}
if (s.s != nullptr)
free(s.s);
return file;
}
inline auto
getline(bgzf_file &file, kstring_t &line) -> bgzf_file & {
if (file.f == nullptr)
return file;
const int x = bgzf_getline(file.f, '\n', &line);
if (x == -1) {
file.destroy();
free(line.s);
line = {0, 0, nullptr};
}
if (x < -1) {
// ADS: this is an error condition and should be handled
// differently from the EOF above. Probably an exception is the
// wrong way to do this.
file.destroy();
free(line.s);
line = {0, 0, nullptr};
throw std::runtime_error{"failed reading bgzf file"};
}
return file;
}
struct bam_tpool {
explicit bam_tpool(const int n_threads)
: tpool{hts_tpool_init(n_threads), 0} {}
~bam_tpool() { hts_tpool_destroy(tpool.pool); }
template <class T>
auto
set_io(const T &bam_file) -> void {
const int ret = hts_set_thread_pool(bam_file.f, &tpool);
// ADS: (todo) get rid of exception
if (ret < 0)
throw std::runtime_error("failed to set thread pool");
}
auto
set_io(const bgzf_file &bgzf) -> void {
const int ret = bgzf_thread_pool(bgzf.f, tpool.pool, tpool.qsize);
if (ret < 0)
throw std::runtime_error("failed to set thread pool");
}
htsThreadPool tpool{};
};
} // namespace bamxx
#endif