Thank you @rrwick for a great tool.
I was wondering what the reason behind the --kmers parameter.
I have samples which were sequenced twice, the second time was done to increase the sequencing depth. Unfortunately the second run was done with a different machine and a different read length.
When I try to run unicycler on the merged fastq files i it fails when it tries to calculate kmers of length 61. the older fastq file is only 60nt long.
Does it make more sense to give the too a specific list of parameters e.g. --kmers 13,25,33,39,45,49,53,57 or is it better to work with the files separately
thanks
Thank you @rrwick for a great tool.
I was wondering what the reason behind the
--kmersparameter.I have samples which were sequenced twice, the second time was done to increase the sequencing depth. Unfortunately the second run was done with a different machine and a different read length.
When I try to run
unicycleron the merged fastq files i it fails when it tries to calculatekmersof length 61. the older fastq file is only 60nt long.Does it make more sense to give the too a specific list of parameters e.g.
--kmers 13,25,33,39,45,49,53,57or is it better to work with the files separatelythanks