@@ -59,7 +59,7 @@ In theory, this workflow could be simplified using `SCPreProcess`;
5959however, due to the performance issue reported in [ Seurat
6060\# 10153] ( https://github.com/satijalab/seurat/issues/10153 ) —where
6161` SCTransform ` hangs or slows down significantly when called via
62- ` do.call ` (as ` SCPreProcess ` does internally)—we instead use a custom
62+ ` do.call ` (as ` SCPreProc ess ` does internally)—we instead use a custom
6363workflow here to avoid the slowdown.
6464
6565(It hasn’t been fixed yet. If it gets resolved, please kindly notify me
@@ -213,7 +213,9 @@ We can now run the screening. Let’s try `Scissor`.
213213
214214A new file named ` Scissor_inputs.RData ` will be created, which contains
215215the input data for the Scissor algorithm. You can use the intermediate
216- data for repeated runs. This is an inherent feature of the ` Scissor ` .
216+ data for repeated runs to save time when tuning parameters, avoiding the
217+ need to re-run the entire pipeline from scratch. This is an inherent
218+ feature of the ` Scissor ` .
217219
218220A new column named ` Scissor ` will be added to the ` meta.data ` of the
219221Seurat object, with three possible labels: ** Positive** , ** Negative** ,
@@ -222,7 +224,8 @@ survival prognosis**.
222224
223225## Visualization of Screened Cells
224226
225- Finally we can visualize the results.
227+ Finally we can visualize the results. Here, we provide a brief
228+ demostration using Seurat’s built-in visualization functions.
226229
227230Let’s first see the spatial position of the Positive cells.
228231
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