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vignettes/Spatial_Transcriptome.md

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@@ -59,7 +59,7 @@ In theory, this workflow could be simplified using `SCPreProcess`;
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however, due to the performance issue reported in [Seurat
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\#10153](https://github.com/satijalab/seurat/issues/10153)—where
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`SCTransform` hangs or slows down significantly when called via
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`do.call` (as `SCPreProcess` does internally)—we instead use a custom
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`do.call` (as `SCPreProc ess` does internally)—we instead use a custom
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workflow here to avoid the slowdown.
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(It hasn’t been fixed yet. If it gets resolved, please kindly notify me
@@ -213,7 +213,9 @@ We can now run the screening. Let’s try `Scissor`.
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A new file named `Scissor_inputs.RData` will be created, which contains
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the input data for the Scissor algorithm. You can use the intermediate
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data for repeated runs. This is an inherent feature of the `Scissor`.
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data for repeated runs to save time when tuning parameters, avoiding the
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need to re-run the entire pipeline from scratch. This is an inherent
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feature of the `Scissor`.
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A new column named `Scissor` will be added to the `meta.data` of the
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Seurat object, with three possible labels: **Positive**, **Negative**,
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## Visualization of Screened Cells
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Finally we can visualize the results.
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Finally we can visualize the results. Here, we provide a brief
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demostration using Seurat’s built-in visualization functions.
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Let’s first see the spatial position of the Positive cells.
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